Journal
PHARMACOLOGICAL RESEARCH
Volume 65, Issue 2, Pages 239-246Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.phrs.2011.11.006
Keywords
Hydrogen sulfide; Ca2+ channel; Rat cerebral artery; Vasodilatation; L-cysteine
Categories
Funding
- National Basic Research Program of China [2012CB517805]
- Hong Kong GRF
- CUHK
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H2S, a gaseous signalling molecule, relaxes blood vessels partly through activation of ATP-sensitive K+ channels. It is however unclear whether H2S or its donors could affect other ion transporting proteins. The present study examined the hypothesis that NaHS, a H2S donor inhibits voltage-sensitive Ca2+ channels and thus relaxes vascular smooth muscle cells (VSMC) in the cerebral arteries. NaHS dilated cerebral arteries from Sprague-Dawley rats with the same potency against pre-contraction by 5-HT and 60 mmol/L KCl, which were unaffected by several K+ channel blockers, N-G-nitro-L-arginine methyl ester or indomethacin, as assessed in wire myograph under an isometric condition. Likewise, NaHS also dilated cerebral arteries against myogenic constriction in pressurized myograph under an isobaric condition. NaHS concentration-dependently inhibited CaCl2-induced contraction in Ca2+-free, 60 mM K+-containing Krebs solution. Patch clamp recordings showed that NaHS reduced the amplitude of L-type Ca2+ currents in single myocytes isolated enzymatically from the cerebral artery. Calcium fluorescent imaging using fluo-4 showed a reduced [Ca2+](i) in 60 mmol/L KCl-stimulated rat cerebral arteries in response to NaHS. H2S precursor L-cysteine-induced relaxation in cerebral arteries was inhibited by cystathionine gamma-lyase (CSE) inhibitor DL-propargylglycine. CSE was expressed in cerebral arteries. In summary, NaHS dilates rat cerebral arteries by reducing L-type Ca2+ currents and suppressing [Ca2+](i) of arterial myocyte, indicating that NaHS relaxes cerebral arteries primarily through inhibiting Ca2+ influx via Ca2+ channels (C) 2011 Elsevier Ltd. All rights reserved.
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