Journal
PHARMACOGNOSY MAGAZINE
Volume 10, Issue 40, Pages 470-476Publisher
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/0973-1296.141819
Keywords
Chromatographic fingerprinting; high-performance liquid chromatography coupled with photodiode array detector; quality control; traditional Chinese medicine; Zhizhu Wan
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Funding
- Key Program of Natural Science Foundation of State [90709019, 81173500, 81302905, 81102556, 81202639]
- Key Science and Technology Program of Heilongjiang Province, China [GC06C501, GA08C303, GA06C30101]
- National Key Technology Research and Development Program of the Ministry of Science and Technology of China [2011BAI03B03, 2011BAI03B06, 2011BAI03B08]
- Foundation of Heilongjiang University of Chinese Medicine [201209]
- National Key Subject of Drug Innovation [2009ZX09502-005]
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Background: Traditional Chinese medicine (TCM) formula has been used for over 1000 years and most of them contain complicate chemical constituents. Chromatographic fingerprinting has been widely accepted as a crucial method for qualitative and quantitative analyses for TCM. Zhi Zhu Wan (ZZW), a classical Chinese medical formula, has been commonly used for the treatment of gastrointestinal disease, which pose a serious challenge to its quality control. Materials and Methods: In this work, a sensitive and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-PDA) was developed to control the quality of ZZW for chemical fingerprint analysis and quantitative analysis of four major bioactive constituents, including hesperidin, naringin, neohesperidin, and atractylenolide I. The chromatographic separation was performed on a Waters Symmetry C18 column (4.6 mm x 250 mm, 5 m particle size), with an aqueous 0.095% phosphate acid and acetonitrile mobile phase gradient. Results: Optimization of other experimental conditions was validated with satisfactory accuracy, precision, repeatability, and recovery. In quantitative analysis, the four components showed good regression (R > 0.9994) within test ranges, and the recovery method ranged from 99.32% to 100.630%. HPLC fingerprints of the ZZW samples were compared by performing similarity analysis. Conclusion: The results indicated that the newly developed HPLC-PDA fingerprint method would be suitable for quality control of ZZW.
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