Journal
PHARMACOGENETICS AND GENOMICS
Volume 20, Issue 6, Pages 396-400Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/FPC.0b013e32833997b0
Keywords
ATP-binding cassette transporter; liquid chromatography/mass spectrometry/mass spectrometry; membrane proteins; platelets; TaqMan low-density array; targeted proteomics
Funding
- Deutsche Forschungsgemeinschaft (DFG) [SFB-TR19]
- German Federal Ministry for Education and Research (BMBF) [03IP612]
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We have identified the ATP-binding cassette (ABC) transporter ABCC4 as an active constituent of mediator-storing granules in human platelets. In addition to multidrug resistance protein 4, other ABC-type transport proteins may contribute to platelet secretory function as well as determine intended or adverse effects of drugs. Here, we provide a comprehensive expression profiling of ABC transporters in human platelets based on a novel screening approach by combining the TaqMan low-density array RNA screening platform with a recently developed liquid chromatography/mass spectrometry (MS)/MS method for the simultaneous detection of membrane proteins. Transcripts of 25 ABC transporters were detected and showed differential expression compared with megakaryocytic progenitor cells. On the protein level ABCA7, ABCB4, ABCC1, ABCC3 and ABCC4 were identified by liquid chromatography/MS/MS and localized by immunofluorescence microscopy. Their functions may be related to glutathione and lipid homeostasis, secretion of lipid mediators, cell protection as well as drug transport. Pharmacogenetics and Genomics 20: 396-400 (C) 2010 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
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