Journal
TUMOR BIOLOGY
Volume 37, Issue 1, Pages 1261-1269Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1007/s13277-015-3921-1
Keywords
miR-20a; NF kappa B; NFKBIB; Cisplatin resistance
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Funding
- National Natural Science Foundation of China [81201705, 81201796]
- Natural Science Foundation of Jiangsu Province [BK2012442]
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Drug resistance of cancer cells can be regulated by the dysregulated miRNAs, and sustained NF kappa B activation also plays an important role in tumor resistance to chemotherapy. Here, we sought to investigate whether there was a correlation between miR-20a and the NF kappa B pathway to clarify the effects that miR-20a exerted on gastric cancer (GC) chemoresistance. We found that miR-20a was significantly upregulated in GC plasma and tissue samples. In addition, it was upregulated in GC plasma and tissues from patients with cisplatin-resistant gastric cancer cell line SGC7901/cisplatin (DDP). And the upregulation of miR-20a was concurrent with the downregulation of NFKBIB (also known as I kappa B beta) as well as upregulation of p65, livin, and survivin. The luciferase activity suggested that NFKBIB was the direct target gene of miR-20a. Transfection of miR-20a inhibitor could increase NFKBIB level; downregulate the expression of p65, livin, and survivin; and lead to a higher proportion of apoptotic cells in SGC7901/DDP cells. Conversely, ectopic expression of miR-20a dramatically decreased the expression of NFKBIB; increased the expression of p65, livin, and survivin; and resulted in a decrease in the apoptosis induced by DDP in SGC7901 cells. Taken together, our findings suggested that miR-20a could promote activation of the NF kappa B pathway and downstream targets livin and survivin by targeting NFKBIB, which potentially contributed to GC chemoresistance.
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