Journal
PHARMACEUTICAL RESEARCH
Volume 29, Issue 5, Pages 1328-1343Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-011-0632-1
Keywords
astrocytes; cell transplantation; iron oxide nanoparticles; multifunctional nanoparticles; neural injury
Funding
- British Biotechnology and Biological Sciences Research Council
- EPSRC [EP/C511794]
- BBSRC [BB/F013884/1, BB/F011350/1] Funding Source: UKRI
- EPSRC [EP/H046143/1, EP/H000925/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F011350/1, BB/F013884/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/C511794/1, EP/H000925/1, EP/H046143/1] Funding Source: researchfish
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To develop Fe3O4-PEI-RITC magnetic nanoparticles with multimodal MRI-fluorescence imaging and transfection capability, for use in neural cell replacement therapies. The Fe3O4-PEI-RITC MNPs were synthesised through a multi-step chemical grafting procedure: (i) Silanisation of MNPs with 3-iodopropyltrimethoxysilane; (ii) PEI coupling with iodopropyl groups on the MNP surface; and (iii) RITC binding onto the PEI coating. The cell labelling and transfection capabilities of these particles were evaluated in astrocytes derived from primary cultures. Fe3O4-PEI-RITC MNPs did not exert acute toxic effects in astrocytes (at a parts per thousand currency sign6 days). Cells showed rapid and extensive particle uptake with up to 100% cellular labelling observed by 24 h. MRI and microscopy studies demonstrate that the particles have potential for use in bimodal MR-fluorescence imaging. Additionally, the particles were capable of delivering plasmids encoding reporter protein (approximately 4 kb) to astrocytes, albeit with low efficiencies. Multifunctional Fe3O4-PEI-RITC MNPs were successfully prepared using a multi-step synthetic pathway, with the PEI and RITC chemically bound onto the MNP surface. Their combined MR-fluorescence imaging capabilities with additional potential for transfection applications can provide a powerful tool, after further development, for non-invasive cell tracking and gene transfer to neural transplant populations.
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