Journal
PHARMACEUTICAL RESEARCH
Volume 28, Issue 8, Pages 1843-1858Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11095-010-0364-7
Keywords
delivery; gene therapy; membrane crossing; nanoparticles; neurons
Funding
- Fondo de Investigaciones Sanitarias [PI52112, PI081434]
- Consejeria de Educacion, JCCM [PII1I09-0163-4002, POII10-0274-3182]
- Ministerio de Ciencia e Innovacion (Spain)
Ask authors/readers for more resources
Efficient methods for cell line transfection are well described, but, for primary neurons, a high-yield method different from those relying on viral vectors is lacking. Viral transfection has several drawbacks, such as the complexity of vector preparation, safety concerns, and the generation of immune and inflammatory responses when used in vivo. However, one of the main problems for the use of non-viral gene vectors for neuronal transfection is their low efficiency when compared with viral vectors. Transgene expression, or siRNA delivery mediated by non-viral vectors, is the result of multiple processes related to cellular membrane crossing, intracellular traffic, and/or nuclear delivery of the genetic material cargo. This review will deal with the barriers that different nanoparticles (cationic lipids, polyethyleneimine, dendrimers and carbon nanotubes) must overcome to efficiently deliver their cargo to central nervous system cells, including internalization into the neurons, interaction with intracellular organelles such as lysosomes, and transport across the nuclear membrane of the neuron in the case of DNA transfection. Furthermore, when used in vivo, the nanoparticles should efficiently cross the blood-brain barrier to reach the target cells in the brain.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available