Separation of positional isomers using chiral chromatography columns

The possibility of using Nucleodex beta-PM and Chiralcel OJ-RH chiral columns for the separation of isomers of butylhydroxyanisole and isosorbide mononitrate was studied. Chromatography over the Chiralcel OJ-RH column (150 x 4.6 mm, 5 mu M) using mobile phases MeOH:H2O (80:20) and CH3CN:H2O (70:30) provided the complete separation of the 3-and 2-isomers of butylhydroxyanisole. In contrast, the separation of isosorbide mononitrate isomers over chiral columns of Nucleodex beta-PM and Chiralcel OJ-RH was not as effective. The observed separations of the 2-and 5-isomers of isosorbide mononitrate were less than over C18 and C10CN columns. Only the ability in principle of using a Nucleodex beta-PM column to separate isosorbide mononitrate isomers was demonstrated.