Journal
PHARMACEUTICAL BIOLOGY
Volume 53, Issue 3, Pages 446-450Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/13880209.2014.923006
Keywords
Amoora rohituka; DNA barcoding; flavopiridol
Funding
- CSIR-Senior research fellowship
- DST-INSPIRE fellowship
- CSIR 12th FYP project 'PMSI' of Council of Scientific and Industrial Research (CSIR) [BSC0117]
- J&K State S T Council [31-ST (30-03-2011)]
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Context: Rohitukine is an important precursor for the synthesis of potential anticancer drugs flavopiridol (Sanofi-Aventis) and P-276-00 (Piramal Healthcare Limited, Mumbai, India). Trunk bark of Dysoxylum binectariferum (Roxb.) Hook. f. ex Bedd. (Meliaceae) is the widely used source for isolation of rohitukine. However, removal of trunk bark threatens the survival of the tree. Objective: To investigate the amount of rohitukine accumulated in other tissues of D. binectariferum. Materials and methods: Rohitukine standard was isolated from leaves of D. binectariferum. Its purity was ascertained using HR-MS and NMR. Crude extracts were prepared from different tissues of D. binectariferum. Rohitukine content in all the tissues was quantified by HPLC. Results: Rohitukine accumulates in a significant amount in seeds, trunk bark, leaves, twigs, and fruits of D. binectariferum. Seeds have the highest rohitukine content (2.42%, dry weight) followed by trunk bark (1.34%, dry weight), leaves (1.064%, dry weight), twigs (0.844% dry weight), and fruits (0.4559% dry weight). Discussion and conclusion: Seeds and leaves of D. binectariferum could be used as alternate renewable sources for isolation of rohitukine.
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