Journal
PHARMACEUTICAL BIOLOGY
Volume 52, Issue 3, Pages 321-329Publisher
INFORMA HEALTHCARE
DOI: 10.3109/13880209.2013.836544
Keywords
Andrographis paniculata; method development and validation; tissue distribution
Funding
- Government of India - AICTE (RPS)
- University Grants Commission (UPE)
Ask authors/readers for more resources
Context: Andrographis paniculata (Burm.f.) Nees (Acanthaceae) is widely used in tribal medicine in India and some other countries for multiple clinical applications. It contains andrographolide (AG) (diterpenoid lactone), a major phytomarker which probably accounts for its medicinal properties. Objective: This study investigates the site-specific distribution of AG in different tissues of rats and its pharmacokinetic parameter evaluation by using a validated liquid chromatography tandem mass spectrometry (LC-MS/MS) method. Materials and methods: A simple and sensitive LC-MS/MS method has been developed and validated to quantify the presence of AG in plasma and various tissues of rat following oral administration of A. paniculata extract and AG in a dose of 133.33 and 100 mg/kg/day, respectively, for four weeks. Results: The present study showed that the highest concentration of AG was in kidney (156.12 ng/g) followed by liver, spleen and brain while almost same concentration was found in heart and lung. The apparent C-max, T-max, elimination half-life and total exposure (AUC(0-alpha)) were 115.81 ng/ml, 0.75, 2.45 and 278.44 ngh/ml, respectively. Conclusion: This was an attempt to determine the presence of AG (a known biomarker) in tissues such as kidney, heart, lungs, brain and plasma of rats using a validated LC-MS/MS method. Furthermore, the observed reduced concentration in plasma and various tissues from 1 to 8 h might be attributed to relatively rapid elimination or distribution of AG from the central compartment.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available