Journal
PHARMACEUTICAL BIOLOGY
Volume 46, Issue 6, Pages 418-424Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880200802055883
Keywords
ATP; cistanche deserticola; H9c2 cells; heart; mitochondria
Ask authors/readers for more resources
To investigate the pharmacological basis of the Yang-invigorating action of Herbal Cistanche [the dried whole plant of Cistanche deserticola Y.C. Ma (Orobanchaceae)], in Chinese medicine, the effects of the methanol extract of Herba Cistanche on mitochondrial ATP generation capacity were examined using an ex vivo rat heart model and an in situ H9c2 cell assay. Treatment with Herba Cistanche extract increased the myocardial mitochondrial ATP generation capacity in a dose-dependent manner in rats, as assessed by in vitro measurement. The stimulation of ATP generation capacity was associated with a parallel enhancement in mitochondrial electron transport supported by pyruvate but not succinate. Herba Cistanche treatment also increased the myocardial mitochondrial complex I and complex III activities, with the extent of stimulation on complex I activity being larger. Herba Cistanche treatment produced a dose-and time-dependent increase in mitochondrial ATP generation capacity in H9c2 cells. The results indicate that Herba Cistanche treatment can increase mitochondrial ATP generation in rat hearts ex vivo and H9c2 cells in situ, possibly through enhancing the oxidative phosphorylation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available