Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 99, Issue 1, Pages 61-64Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2010.10.006
Keywords
DNA damage; Dexon; Mitotic index; Cornet assay
Categories
Funding
- Afyon Kocatepe University Scientific Research Committee [07.FENED.23]
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Genotoxic effects of Fenaminosulf, fungicide and micro-biocide, were examined by using mitotic index (MI), mitotic phase, and Comet assay on the root meristem cells of Allium cepa. In the Album root growth inhibition test, EC50 value was firstly determined as 25 ppm and, 12.5 (0.5 x EC50), 25 (EC50) and 50 (2 x EC50) ppm concentrations of Fenaminosulf were introduced to onion tuber roots. Distilled water was used as a negative control. All obtained data were subjected to statistical analyses by using SPSS 15.0 for Windows software. For comparison purposes, Duncan multiple range tests by using one-way analysis of variance (ANOVA) were employed and p < 0.05 was accepted as significant value. While MI (except in 24 h at 12.5 and 50 ppm) and prophase index increased, metaphase, anaphase and telophase indexes decreased in all concentrations compared to control at each exposure time. A significant increase in DNA damage was also observed at the concentration of 25 ppm in 24 h, 25 and 50 ppm in 96 h by Comet assay. (C) 2010 Elsevier Inc. All rights reserved.
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