Protective role of propolis against reproductive toxicity of chlorpyrifos in male rats

Chlorpyrifos (CPF) is causing different toxicity and propolis has been reported to be an effective antioxidant. Therefore, the present study is aimed to elucidate the possible protective effects of propolis in alleviating the toxicity of chlorpyrifos on reproductive performance, testosterone levels, enzyme activities and lipids profile in serum of male rats. Animals were divided into four groups; one orally administered (o.d.) CPF at a dose of 9 mg/kg b.w. for consecutive 70-days, propolis group (50 mg/kg b.w.), propolis and chlorpyrifos group and a control group. Results showed that there was a correlation between CPF administration and the significant decrease of the sperm counts, spermatozoon survival and testosterone level as well as increase of sperm aberrations. CPF increased significantly the lipid profile and the levels of various serum liver marker enzymes. In contrast, co-administration of propolis to CPF-treated rats restored almost most of these biochemical parameters to normal levels. On the other hand, CPF resulted in histopathological alterations in testes of male rats. However, pre-administration of propolis to CPF-treated animals improved the testicular damage and alleviates the toxic effects of CPF on reproductive functions in male rats. (C) 2011 Elsevier Inc. All rights reserved.