Journal
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
Volume 98, Issue 2, Pages 206-212Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.pestbp.2010.06.008
Keywords
Aminocarb; Calf thymus DNA; Spectroscopy; Binding mode; Thermodynamic parameter
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Funding
- Program of Science and Technology of Jiangxi Province [2009BNA09000]
- Research Program of State Key Laboratory of Food Science and Technology of Nanchang University [SKLF-TS-200917, SKLF-MB-200807]
- Program of Science and Technology of General Administration of Quality Supervision, Inspection and Quarantine of China [2009IK141]
- Foundation of Analytical and Test of Nanchang University [2008006]
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The interaction between aminocarb and calf thymus DNA in physiological buffer (pH 7.4) was investigated by UV-vis spectrophotometry, fluorescence spectroscopy, viscosity measurements and DNA melting techniques. The absorption spectra of aminocarb with DNA showed a slight blue shift and hypochromic effect. Using ethidium bromide (EB) as a fluorescence probe, fluorescence quenching of the emission peak was seen in the DNA-EB system when aminocarb was added. The fluorescence polarization was gradually increased with increasing amounts of DNA. The value of melting temperature of DNA increased in the presence of aminocarb. Moreover, the relative viscosity of DNA increased with the addition of aminocarb. All the evidences indicated that the binding mode of aminocarb with DNA was an intercalative binding. The binding constants of aminocarb with DNA were determined. The calculated thermodynamic parameters suggested that the binding of aminocarb to DNA was driven mainly by hydrogen bond and van der Waals. (C) 2010 Elsevier Inc. All rights reserved.
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