Biological activity of the essential oil from the leaves of Piper sarmentosum Roxb. (Piperaceae) and its chemical constituents on Brontispa longissima (Gestro) (Coleoptera: Hispidae)

The results showed that the essential oil from Piper sarmentosum has strong antifeedant and toxicity effects on Brontispa longissima The best antifeedant and contact toxicity effects were observed in the 1st-2nd instar larvae. The essential oil also displayed a notable fumigation effect on the eggs and pupae of B longissima It took the control-treated insects 43 34 d to complete one generation, while the insects treated with 2000 mg/L essential oil needed 73 58 d The essential oil was analyzed by gas chromatography/mass spectrometry and 41 components were identified Myristicin (65 22%) and trans-caryophyllene 13 89%) were the major components Myristicin exhibited strong antifeedant and contact toxicity effects on both the 3rd instar larvae and the imagoes of B longissima, when it showed a significant fumigation effect on the eggs and pupae of B longissima Another P sarmentosum essential oil and myristicin all showed a strong inhibiting effect on the growth and development of B longissima along with the activity of AChE, CarE, GSTs and Na+. K+-ATPase in B longissima larvae (C) 2009 Elsevier Inc All rights reserved.