Journal
PEST MANAGEMENT SCIENCE
Volume 70, Issue 10, Pages 1588-1594Publisher
JOHN WILEY & SONS LTD
DOI: 10.1002/ps.3751
Keywords
Bemisia tabaci; detoxification enzymes; quantitative real-time PCR analysis; cytochrome-P450-dependent monooxygenase; carboxylesterase; glutathione S-transferase
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Funding
- National Science Fund for Distinguished Young Scholars [31025020]
- 863 Programme [2012AA101502]
- National Science and Technology Pillar Programme [2012BAD19B01]
- Special Fund for Agroscientific Research in the Public Interest [201203038]
- Beijing Key Laboratory for Pest Control and Sustainable Cultivation of Vegetables
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BACKGROUND: The sweetpotato whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), is one of the most invasive and destructive pests of field crops worldwide. The sibling species B and Q are the two most damaging members of the B. tabaci species complex. That Q is more resistant than B to many insecticides has been well documented. Over the last decade, Q has gradually displaced B and has become the dominant form of B. tabaci in field agricultural systems inmost parts of China. To help understand the differences in insecticide resistance, the activities and gene expression profiles of detoxification enzymes in B. tabaci B and Q were investigated. RESULTS: The activity of P450 towards 7-ethoxycoumarin was significantly higher (1.46-fold higher) in Q than in B. The expression of 43 of 65 P450 genes was higher (>1-fold) in Q than in B, and expression for eight P450 genes was more than 50-fold greater in Q than in B. The increased expression of selected P450 genes in Q relative to B was confirmed with two other B strains and two other Q strains. On the other hand, carboxylesterase (CarE) activity was significantly lower (0.71-fold lower) in Q than in B; the K-m value of CarE was significantly lower in B than in Q, but the opposite was true for the V-max value of CarE. Glutathione S-transferase activity and values of K-m and V-max did not differ between B and Q. CONCLUSION: Enhanced metabolic detoxification of insecticides by P450s may be an important reason why B. tabaci Q is more resistant than B. tabaci B to insecticides. (C) 2014 Society of Chemical Industry
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