Journal
PESQUISA VETERINARIA BRASILEIRA
Volume 32, Issue 8, Pages 692-696Publisher
REVISTA PESQUISA VETERINARIA BRASILEIRA
DOI: 10.1590/S0100-736X2012000800002
Keywords
Mastitis; coagulase negative staphylococci; antimicrobial resistance; gene mecA; gene blaZ; cattle
Categories
Funding
- CNPq
- FAPERJ [E-26/171.360/2005, Proc. E-26/171.366/2006, E-26/110.910/2008]
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Soares L. C., Pereira I. A., Pribul B. R., Oliva M. S., Coelho S. M. O. & Souza M. M. S. 2012. Antimicrobial resistance and detection of mec and blaZ genes in coagulase-negative Staphylococcus isolated from bovine mastitis. Pesquisa Veterinaria Brasileira 32(8): 692-696. Departamento de Microbiologia Veterinaria, Instituto de Veterinaria, Universidade Federal Rural do Rio de Janeiro, BR 465 Km 7, Seropedica, RJ 23890-000, Brazil. E-mail: miliane@ufrrj.br The present study evaluated the pheno- and genotypical antimicrobial resistance profile of coagulase-negative Staphylococcus (CNS) species isolated from dairy cows milk, specially concerning to oxacillin. Of 100 CNS isolates, the S. xylosus was the prevalent species, followed by S. cohnii, S. hominis, S. capitis and S. haemolyticus. Only 6% were phenotypically susceptible to the antimicrobial agents tested in disk diffusion assay. Penicillin and ampicillin resistance rates were significantly higher than others antimicrobials. Four isolates were positive to mecA gene (4%), all represented by the S. xylosus species. The blaZ gene was detected in 16% of the isolates (16/100). It was noticed that all mecA + were also positive to this gene and the presence of both genes was correlated to phenotypic beta-lactamic resistance. We conclude that CNS species from bovine milk presented significantly distinct antimicrobial resistance profiles, evaluated by phenotypic and genotypic tests, which has implications for treatment and management decisions.
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