Journal
PEPTIDES
Volume 35, Issue 2, Pages 291-299Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2012.03.016
Keywords
Peptide; Molecular cloning; Venom; Scorpion; Antimicrobial
Ask authors/readers for more resources
The venoms of scorpions are complex cocktails of polypeptide toxins that fall into two structural categories: those that contain cysteinyl residues with associated disulfide bridges and those that do not. As the majority of lethal toxins acting upon ion channels fall into the first category, most research has been focused there. Here we report the identification and structural characterization of two novel 18-mer antimicrobial peptides from the venom of the North African scorpion,Androctonus amoreuxi. Named AamAP1 and AamAP2, both peptides are C-terminally amidated and differ in primary structure at just two sites: Leu double right arrow Pro at position 2 and Phe double right arrow Ile at position 17. Synthetic replicates of both peptides exhibited a broad-spectrum of antimicrobial activity against a Gram-positive bacterium (Staphylococcus aureus), a Gram-negative bacterium (Escherichia coli) and a yeast (Candida albicans), at concentrations ranging between 20 mu M and 150 mu M. In this concentration range, both peptides produced significant degrees of hemolysis. A synthetic replicate of AamAP1 containing a single substitution (His double right arrow Lys) at position 8, generated a peptide (AamAP-S1) with enhanced antimicrobial potency (3-5 mu M) against the three test organisms and within this concentration range, hemolytic effects were negligible. In addition, this His double right arrow Lys variant exhibited potent growth inhibitory activity (ID50 25-40 mu m) against several human cancer cell lines and endothelial cells that was absent in both natural peptides. Natural bioactive peptide libraries, such as those that occur in scorpion venoms, thus constitute a unique source of novel lead compounds with drug development potential whose biological properties can be readily manipulated by simple synthetic chemical means. (c) 2012 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available