Journal
PEPTIDES
Volume 32, Issue 6, Pages 1159-1165Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2011.04.008
Keywords
Conotoxin; Conus quercinus; Disulfide connectivity; Mutation; NMR; Electrophysiology
Funding
- National Basic Research Program of China [2010CB529802, 2007CB914304]
- Ministry of Education of China [NCET-10-0604]
- Program of Shanghai Subject Chief Scientist [09XD1405100]
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A novel conotoxin, qc16a, was identified from the venom of vermivorous Conus quercinus. qc16a has only 11 amino acid residues, DCQPCGHNVCC, with a unique cysteine pattern. Its disulfide connectivity was determined to be I-IV, II-III. The NMR structure shows that qc16a adopts a ribbon conformation with a simple beta-turn motif formed by residues Gly6, His7 and Asn8. qc16a causes depression symptom in mice when injected intracranially. Point mutation results showed that Asp1, His7 and Asn8 are all essential for the activity of qc16a. Electrophysiologically, qc16a has no strong effect on the whole-cell currents of neurons and the currents of Drosophila Shaker channels, human BK channels and Na(V)1.7 channels. Its specific target still remains to be identified. (C) 2011 Elsevier Inc. All rights reserved.
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