Journal
PEPTIDES
Volume 29, Issue 9, Pages 1526-1533Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.peptides.2008.05.008
Keywords
amphibian; Pelophylax (Rana) saharica; temporins
Funding
- Centre National de la Recherche Scientifique (CNRS)
- FEBS Short-Term Fellowship
- Tunisian Secretariat of Scientific Research
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Temporins are small antimicrobial peptides isolated from North American and Eurasian ranid frogs that are particularly active against Gram-positive bacteria. To date, no temporins have been characterized from North African frog species. We isolated three novel members of the temporin family, named temporin-1Sa (FLSGIVGMLGKLF(amide)), -1Sb (FLPIVTNLLSGLL(amide)), and -1Sc (FLSHIAGFLSNLF(amide)), from the skin of the Sahara frog Pelophylax (Rana) saharica originating from Tunisia. These temporins were identified by a combined mass spectrometry/molecular cloning approach. Temporin-1Sa was found to be highly active against Gram-positive and Gram-negative bacteria, yeasts and fungi (MIC = 2-30 mu M). To our knowledge, this is the first 13-residue member of the temporin family with a net charge of +2 that shows such broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 10 mu M) and Pseudomonas aeruginosa (MIC = 31 mu M). Moreover, temporin-1Sa displays significant antiparasitic activity (IC50 similar to 20 mu M) against the promastigote and amastigote stages of Leishmania infantum. (C) 2008 Elsevier Inc. All rights reserved.
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