Journal
TRENDS IN BIOTECHNOLOGY
Volume 33, Issue 10, Pages 611-620Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2015.07.005
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Funding
- National Natural Science Foundation of China [31271739, 31471512]
- Project of Transgenic New Variety Cultivation [2014ZX08003-003]
- Raymond F. Baker Center for Plant Breeding of Iowa State University
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Increasing knowledge of plant genome sequences requires the development of more reliable and efficient genetic approaches for genotype-phenotype validation. Functional identification of plant genes is generally achieved by a combination of creating genetic modifications and observing the according phenotype, which begins with forward-genetic methods represented by random physical and chemical mutagenesis and move towards reverse-genetic tools as targeted genome editing. A major bottleneck is time need to produce modified homozygous genotypes that can actually be used for phenotypic validation. Herein, we comprehensively address and compare available experimental approaches for functional validation of plant genes, and propose haploid strategies to reduce the time needed and cost consumed for establishing gene function.
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