Journal
TRENDS IN BIOTECHNOLOGY
Volume 33, Issue 7, Pages 401-407Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2015.04.003
Keywords
long-term culture; tissue engineering; microfluidics; 2D culture; 3D culture; bioreactors
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Funding
- National Institutes of Health (NIH) Tissue Engineering Resource Center [P41 EB002520]
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This review examines important robust methods for sustained, steady-state, in vitro culture. To achieve 'physiologically relevant' tissues in vitro additional complexity must be introduced to provide suitable transport, cell signaling, and matrix support for cells in 3D environments to achieve stable readouts of tissue function. Most tissue engineering systems draw conclusions on tissue functions such as responses to toxins, nutrition, or drugs based on short-term outcomes with in vitro cultures (2-14 days). However, short-term cultures limit insight with physiological relevance because the cells and tissues have not reached a steady-state.
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