Effect of rapid fluid resuscitation using crystalloids or colloids on hemostasis in piglets

Background and Objectives Impairment of blood coagulation is one of the main side effects of volume replacement, particularly if artificial colloids such as hydroxyethyl starch (HES) and gelatine preparations are used. This animal study aimed to evaluate the effect of a single fast intravenous crystalloid or colloid fluid bolus on blood coagulation as measured by rotation thromboelastometry (ROTEM (R)). Methods Thirty-two anesthetized piglets were infused with a rapid 20ml center dot kg1 fluid bolus of either normal saline (NS), 4% gelatine, 5% albumin or 6% HES 130/0.4 (n=8 per group) over a period of 2min. Hemostasis was assessed by ROTEM (R) before and 1min after fluid administration. Within-group differences were analyzed by Wilcoxon test, and additionally overall KruskalWallis test followed by posthoc MannWhitney U-test were applied to detect differences between groups. Results All fluids caused a significant weakening of clot strength within groups. HES and gelatine showed a significantly stronger impairment of clot growth and maximum clot firmness as compared with albumin and normal saline. Impairment of fibrin polymerization was more pronounced following HES as compared with all other fluids. Conclusion After moderate but very fast volume loading, HES and gelatine impair blood coagulation to a larger extent as compared with albumin or normal saline, while no significant differences were observed between both artificial colloids.