Journal
PEDIATRIC ALLERGY AND IMMUNOLOGY
Volume 21, Issue 4, Pages E772-E779Publisher
WILEY
DOI: 10.1111/j.1399-3038.2009.00904.x
Keywords
antibodies; Lactobacillus acidophilus; immunoblot; immunoproteomics; oxalyl-CoA decarboxylase; formyl-CoA transferase
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Funding
- Estonian Science Foundation [6514]
- EU
- Centre of Molecular and Clinical Medicine, University of Tartu
- Proteomics Core Laboratory (Institute of Technology, University of Tartu)
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Immune responses to lactobacilli have been so far insufficiently investigated in patients with autoimmune diseases. We used whole-cell lysate of an indigenous Lactobacillus acidophilus strain isolated from an Estonian child to study serum IgG antibodies in children groups with type 1 diabetes [insulin dependent diabetes mellitus (IDDM)] (n = 21, age 4-18 yr) and with acute coeliac disease (CD) (n = 20, age 0.6-15 yr) and to compare the results with the controls (n = 24, age 2-17 yr). We found that our developed 1-D immunoblot assay readily enables to reveal antibodies against 28 L. acidophilus antigenic proteins in patients' and controls' sera. As verified by immunoproteomics analysis with 2-D and LC ESI-MS/MS the antigens of L. acidophilus were mainly common cytoplasmic proteins GroEL (HSP60), enolase, transcripton factor EF-Ts and EF-Tu. However, in addition we identified formyl-CoA transferase being target for antibodies in every tested IDDM patients' serum. We have characterized for the first time the antigenic profile of L. acidophilus whole-cell lysate using sera from children with IDDM, CD, and controls. The different prevalence of reactions against tested antigens in patients and controls sera may indicate significant differences in immune system and commensal bacteria cross-talk in these groups.
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