Journal
PATHOLOGY & ONCOLOGY RESEARCH
Volume 16, Issue 2, Pages 229-237Publisher
SPRINGER
DOI: 10.1007/s12253-009-9215-4
Keywords
Pim-2; NF-kappa B; API-5; Hepatocellular carcinoma cells; Apoptosis
Funding
- National Natural Scientific Foundation of China [30400424]
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Pim-2 is proved to be relevant to the tumorigenesis of hepatocellular carcinoma (HCC), but the mechanism is unclear. We studied the relationship among Pim-2, NF-kappa B and API-5. In our experiment, expression level of the three factors and phosphorylation level of API-5, as well as NF-kappa B activity, were detected in HCC tissues and the nontumorous controls. Then Pim-2 gene was transfected into nontumorous liver cells L02, and Pim-2 SiRNA was transfected into hepatoblastoma cell line HepG2. Parthenolide was added as NF-kappa B inhibitor. The same detections as above were repeated in the cells, along with the apoptosis analysis. We found the levels of Pim-2, NF-kappa B and API-5, as well as NF-kappa B activity, were significantly higher in HCC tissues. Pim-2 level was increased in L02 cells after the transfection of Pim-2 gene, but decreased in HepG2 cells after the transfection of Pim-2 SiRNA. The levels of NF-kappa B and API-5, as well as NF-kappa B activity and API-5 phosphorylation level, were in accordance with Pim-2 level, but could be reversed by Parthenolide. Cell apoptosis rates were negatively correlated with API-5 phosphorylation level. Therefore, we infer that Pim-2 could activate API-5 to inhibit the apoptosis of liver cells, and NF-kappa B is the key regulator.
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