Journal
PARASITOLOGY INTERNATIONAL
Volume 62, Issue 6, Pages 535-542Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.parint.2013.08.001
Keywords
Bile acids; Flukes; Opisthorchis viverrini; Oxysterols
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Funding
- Fundacao para a Ciencia e Tecnologia (FCT, Portugal)
- FEDER (European Union) [CONC-REEQ/275/QUI, PEst-C/QUI/UI0081/2011]
- FCT [SFRH/BPD/48345/2008, Pest-OE/AGR/UIO211/2011, UI211-2011-2013]
- National Cancer Institute [R01CA155297, R01CA164719]
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Liquid chromatography in tandem mass spectrometry (LC-MS/MS) has emerged as an informative tool to investigate oxysterols (oxidized derivatives of cholesterol) in helminth parasite associated cancers. Here, we used LC-MS/MS to investigate in soluble extracts of the adult developmental stage of Opisthorchis viverrini from experimentally infected hamsters. Using comparisons with known bile acids and the metabolites of estrogens, the LC-MS data indicated the existence of novel oxysterol derivatives in O. viverrini. Most of these derivatives were ramified at C-17, in similar fashion to bile acids and their conjugated salts. Several were compatible with the presence of an estrogen core, and/or hydroxylation of the steroid aromatic ring A, hydroxylation of both C-2 and C-3 of the steroid ring and further oxidation into an estradiol-2,3-quinone. (C) 2013 Published by Elsevier Ireland Ltd.
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