Journal
PARASITOLOGY
Volume 142, Issue 5, Pages 648-659Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182014001711
Keywords
Moniezia; drug metabolism; anthelmintic; benzimidazole; albendazole
Categories
Funding
- Czech Science Foundation (GA CR) [P502/10/0217]
- Grant Agency of Charles University (GA UK) [673612/B-CH/2012]
- Charles University in Prague [SVV 260 065]
- European Social Fund
- state budget of the Czech Republic [CZ.1.07/2.3.00/30.0022]
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The sheep tapeworm Moniezia expansa is very common parasite, which affects ruminants such as sheep, goats as well as other species. The benzimidazole anthelmintics albendazole (ABZ), flubendazole (FLU) and mebendazole (MBZ) are often used to treat the infection. The drug-metabolizing enzymes of helminths may alter the potency of anthelmintic treatment. The aim of our study was to assess the activity of the main drug-metabolizing enzymes and evaluate the metabolism of selected anthelmintics (ABZ, MBZ and FLU) in M. expansa. Activities of biotransformation enzymes were determined in subcellular fractions. Metabolites of the anthelmintics were detected and identified using high performance liquid chromatography/ultra-violet/VIS/fluorescence or ultra-high performance liquid chromatography/mass spectrometry. Reduction of MBZ, FLU and oxidation of ABZ were proved as well as activities of various metabolizing enzymes. Despite the fact that the conjugation enzymes glutathione S-transferase, UDP-glucuronosyl transferase and UDP-glucosyl transferase were active in vitro, no conjugated metabolites of anthelmintics were identified either ex vivo or in vitro. The obtained results indicate that sheep tapeworm is able to deactivate the administered anthelmintics, and thus protects itself against their action.
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