Journal
PARASITOLOGY
Volume 135, Issue 5, Pages 575-583Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0031182008004174
Keywords
Schistosoma; Schistosoma japonicum; microarray; real-time PCR; gene expression
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Funding
- Wellcome Trust [071657] Funding Source: Medline
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Schistosomes are complex parasitic helminths with discrete life-cycle stages, adapted for survival in their mammalian and snail hosts and the external aquatic environment. Recently, we described the fabrication and use of a microarray to investigate gender-specific transcription in Schistosoma japonicum. To address transcriptional differences, 8 gender-associated gene transcripts identified previously by the microarray analysis were selected for further study. First, differential transcription patterns were investigated in 4 developmental stages using real-time PCR. Subsequently, we undertook functional analysis of a subset of 4 transcripts encoding metabolic enzymes, so as to correlate gender-associated transcript levels with enzyme activity in protein extracts from adult worms. The 8 characterized molecules serve as a basis for further investigation of differential gene expression during the schistosome life-cycle and for studying the sexual dimorphism of adult worn-is. Continual refinement and annotation of the microarray used in the current study should support future work Oil these aspects.
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