Journal
PARASITE IMMUNOLOGY
Volume 34, Issue 11, Pages 536-546Publisher
WILEY
DOI: 10.1111/pim.12003
Keywords
autoimmunity; inflammation; rodent; innate immunity; animal model; parasite; Heligmosomoides polygyrus
Categories
Funding
- National Science Centre [N303 819140]
- [POIG.02.02.00-14-024/08-00]
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Primary exposure of mice to the nematode Heligmosomoides polygyrus infection reduces inflammation in an experimental model of colitis. The aim of the present investigation was to evaluate whether the reduced inflammation provoked by H similar to polygyrus L4 larvae in BALB/c mice treated with dextran sulphate sodium is associated with changed expression of opioids in the small intestine and colon. Colitis was induced by 5% Dextran sulphate sodium (DSS) oral administration for 3 similar to days before oral infection with 200 infective larvae (L3) H similar to polygyrus until the end of the experiment, 6 similar to days post-infection. Clinical disease symptoms were monitored daily. The expressions of proopiomelanocortin POMC1, MOR1 (Oprm1) opioid receptor and beta-endorphin were determined by RT-PCR, Western blot and immunoassay, respectively, in the colon and small intestine of mice. RT-PCR analysis of colon tissues showed up-regulation of the expression of POMC and MOR1 opioid-dependent genes in mice with DSS-induced colitis. H.similar to polygyrus L4 larvae inhibited DSS-induced colitis symptoms that were correlated with increased IL-1 beta, TNF-a, IL-6, myeloperoxidase (MPO) concentration, macrophages infiltration and MOR1, POMC and beta-endorphin increased expression in the small intestine and inhibition of those in the colon.
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