Journal
PAIN
Volume 137, Issue 3, Pages 507-519Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1016/j.pain.2007.10.013
Keywords
tibia fracture; neuropathic pain; TNF; sTNF-R1; MicroCT scanning; Fos immunoreactivity; complex regional pain syndrome
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Funding
- NIDDK NIH HHS [R01 DK067197-04, R01 DK067197, DK67197] Funding Source: Medline
- NIGMS NIH HHS [GM65345, R01 GM065345, R01 GM065345-05] Funding Source: Medline
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Tibia fracture in rats initiates a cascade of nociceptive, vascular, and bone changes resembling complex regional pain syndrome type I (CRPS 1), Previous studies suggest that the pathogenesis of these changes is attributable to an exaggerated regional inflammatory response to injury. We postulated that the pro-inflammatory cytokine tumor necrosis factor alpha (TNF) might mediate the development of CRPS-like changes after fracture. RT-PCR and EIA assays were used to evaluate changes in TNF expression and content in skin, nerve, and bone after fracture. Bilateral hindpaw thickness, temperature, and nociceptive thresholds were determined, and bone microarchitecture was measured using microcomputed tomography. Lumbar spinal cord Fos immunostaining was performed for quantification of Fos positive neurons. After baseline testing, the distal tibia was fractured and the hindhillb casted for 4 weeks. The rats were subcutaneously injected either with a soluble TNF receptor type I (sTNF-R1, 5 mg/kg/d) or saline every 3 days over 28 days and then were retested at 4 weeks post-fracture. Tibia fracture chronically upregulated TNF expression and protein levels in the hindpaw skin and sciatic nerve. After fracture the rats developed hindpaw mechanical allodynia and unweighting, which were reversed by sTNF-R1 treatment. Consistent with the behavioral data, spinal Fos increased after fracture and this effect was inhibited by sTNF-R1 treatment. Collectively, these data suggest that facilitated TNF signaling in the hindlimb is an important mediator of chronic regional nociceptive sensitization after fracture, but does not contribute to the hindlimb warmth, edema, and bone loss observed in this CRPS I model. Published by Elsevier B.V. on behalf of International Association for the Study of Pain.
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