Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 70, Issue -, Pages 50-57Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2015.01.017
Keywords
Carbon-based electrode; Cytochrome P450; Electrochemistry; Gold electrode; Metalloporphyrin; Modified electrode; Oxidative drug metabolism; Self-assembly monolayer; Surface modification; Surface-modified electrode
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Funding
- Dutch Technology Foundation STW [11957]
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This review discusses different approaches that have been taken to mimic oxidative drug metabolism as executed by members of the Cytochrome P450 (CYP450) family of enzymes in humans. Non-modified electrodes can be used to produce some of the oxidative drug metabolites observed in vivo but their scope is rather limited. Modifying electrodes with simple cofactors in analogy to those observed in CYP450 but without the protein scaffold extends these possibilities and notably allows driving reactions following a CYP450-like mechanism. The review ends with approaches to immobilize CYP450s or analogs thereof on electrodes to mimic the in vivo drug metabolism fully. We discuss future perspectives with respect to the advantages and the disadvantages of each level of complexity and possible ways forward. (C) 2015 Elsevier B.V. All rights reserved.
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