Journal
TOXICOLOGY IN VITRO
Volume 29, Issue 6, Pages 1268-1274Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2014.12.021
Keywords
In vitro cytotoxicity; Nanoparticles
Categories
Funding
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
- Financiadora de Estudos e Pesquisas (FINEP)
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
- Fundacao de Apoio a Pesquisa da Universidade Federal de Goias (FUNAPE)
- Fundacao de Apoio a Pesquisa do Estado de Goias (FAPEG)
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Nanostructured drug delivery systems are based on biocompatible and biodegradable components. Composition, size and membrane surface properties are characteristics that may influence cell viability in cytotoxicity assays. In this work, four nanostructured systems commonly used for drug delivery were prepared and cytotoxicity was evaluated on human lymphocytes and Balb/c 3T3 fibroblasts. The hemolytic potential was also investigated. Polymeric nanocapsules (NC) and nanospheres (NS), nanostructured lipid carriers (NLC) and liposomes were prepared and characterized for size, distribution, zeta potential and number per volume of the colloidal dispersion. Cell viability was evaluated, 24 and 48 h, by MTT and neutral red assays (NR). Cells were incubated with each particle in eight different dilutions varying from 2.1 x 10(4) to 2.1 x 10(11) particles/mL. Diameter of nanoparticles was between 130 and 200 nm, all samples exhibited narrow size distribution (polydispersity index below 0.1) and zeta potential varied from -6.8 to -19.5 mV. NC, NS and NLC reduced cell viability in a dilution dependent manner. For these nanoparticles, the higher number of particles induced cell death for both cell types. Liposomes did not cause loss of cell viability even at the highest number of particles. Results suggest that, depending on the kind of nanoparticle, the number of particles in the dispersion can negatively influence cell viability in pre-clinical drug development. (C) 2015 Elsevier Ltd. All rights reserved.
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