Journal
TOXICOLOGY AND APPLIED PHARMACOLOGY
Volume 282, Issue 1, Pages 52-60Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.taap.2014.11.004
Keywords
Aflatoxin B-1; Sulforaphane; Chemoprevention; Membrane biosynthesis; Gene expression profiling
Categories
Funding
- National Institutes of Health [ES016313, P30-ES002109, P01 ES006052, P30 ES003819, P30 CA006973]
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Aflatoxin B-1 (AFB(1)) is one of the major risk factors for liver cancer globally. A recent study showed that sulforaphane (SF), a potent inducer of phase II enzymes that occurs naturally in widely consumed vegetables, effectively induces hepatic glutathione S-transferases (GSTs) and reduces levels of hepatic AFB(1)-DNA adducts in AFB(1)-exposed Sprague Dawley rats. The present study characterized the effects of SF pretreatment on global gene expression in the livers of similarly treated male rats. Combined treatment with AFB(1) and SF caused reprogramming of a network of genes involved in signal transduction and transcription. Changes in gene regulation were observable 4 h after AFB(1) administration in SF-pretreated animals and may reflect regeneration of cells in the wake of AFB(1)-induced hepatotoxicity. At 24 h after AFB(1) administration, significant induction of genes that play roles in cellular lipid metabolism and acetyl-CoA biosynthesis was detected in SF-pretreated AFB(1)-dosed rats. Induction of this group of genes may indicate a metabolic shift toward glycolysis and fatty acid synthesis to generate and maintain pools of intermediate molecules required for tissue repair, cell growth and compensatory hepatic cell proliferation. Collectively, gene expression data from this study provide insights into molecular mechanisms underlying the protective effects of SF against AFB(1) hepatotoxicity and hepatocarcinogenicity, in addition to the chemopreventive activity of this compound as a GST inducer. (C) 2014 Elsevier Inc. All rights reserved.
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