Journal
OSTEOARTHRITIS AND CARTILAGE
Volume 18, Issue -, Pages S113-S116Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.joca.2010.05.026
Keywords
Cartilage; Osteoarthritis histochemistry; Toluidine blue; Safranin O; Picrosirius red Goldner's trichrome stain hematoxylin-eosine
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Funding
- Bayer
- Expanscience
- Genzyme
- Lilly
- MerckSerono
- Novartis
- Pfizer
- SanofiAventis
- Servier
- Wyeth
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Histological and histochemical methods are important tools in the evaluation of joint tissue samples for degenerative joint diseases, both in humans and in animal models In this respect. standardized, simple, and reliable techniques are mandatory This chapter describes five basic staining procedures appropriate for macroscopic (Indian ink) and histologic (HE/hematoxylin - eosin) visualization and scoring of cartilage proteoglycan and collagen content (toluidine blue/safranin O and picrosirius red/Goldner's trichrome) (C) 2010 Osteoarthritis Research Society International Published by Elsevier Ltd. All rights reserved
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