Journal
ORIGINS OF LIFE AND EVOLUTION OF BIOSPHERES
Volume 44, Issue 4, Pages 331-334Publisher
SPRINGER
DOI: 10.1007/s11084-014-9389-y
Keywords
Cell-free protein synthesis system; Liposome; Membrane protein; SecYEG translocon
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Funding
- Grants-in-Aid for Scientific Research [26119704, 26103511, 26660082, 26106003] Funding Source: KAKEN
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The cell membrane has many indispensable functions for sustaining cell alive besides a role as merely outer envelope. The most of such functions are implemented by membrane embedded proteins that are emerged through the membrane integration machinery, SecYEG translocon. Here, we synthesized SecYEG by expressing the corresponding gene in vitro to study the process of functionalization of the cell membrane.
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