Development of an Improved Immobilized CAL-B for the Enzymatic Resolution of a Key Intermediate to Odanacatib

An immobilized form of Candida antarctica lipase B (CAL-B) has been developed with enhanced stability and activity compared to commercially available preparations. The immobilized CAL-B is more active, and 15 times more stable than the previously used preparation. This permits a continuous dynamic kinetic resolution process that is significantly less expensive than the original batch process, and with a 3-fold reduction in the process E-factor.