Journal
ORGANIC LETTERS
Volume 16, Issue 23, Pages 6092-6095Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ol502712v
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Funding
- FAPESP (Sao Paulo Research Foundation) [2013/04540-0]
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E. coli cells containing overexpressed (R)-selective omega-transaminase and the cofactor PLP were immobilized on methacrylate beads suitable for continuous flow applications. The use of an organic solvent suppresses leaching of PLP from the cells; no additional cofactor was required after setting up the packed-bed reactor containing the biocatalyst (omega-TA-PLP). Non-natural ketone substrates were transformed in flow with excellent enantioselectivity (>99% ee). Features of this novel system include high-throughput (30-60 min residence time), clean production (no quench, workup, or purification required), high enzyme stability (the packed-bed reactor can be continuously operated for 1-10 days), and excellent mass recovery.
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