Journal
ORGANIC LETTERS
Volume 13, Issue 16, Pages 4156-4159Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ol201073e
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Funding
- NIH [GM095086]
- NSF [CHE-0750235, MCB-0824837]
- Georgia Cancer Coalition (GCC) Distinguished Cancer Clinicians and Scientists
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [0824837] Funding Source: National Science Foundation
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By differentiating the functional groups on nucleosides, we have designed and developed a one-pot synthesis of deoxyribonucleoside 5'-triphosphates without any protection on the nucleosides. A facile synthesis is achieved by generating an in situ phosphitylating reagent that reacts selectively with the 5'-hydroxyl groups of the unprotected nucleosides. The synthesized triphosphates are of high quality and can be effectively incorporated into DNAs by DNA polymerase. This novel approach is straightforward and cost-effective for triphosphate synthesis.
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