4.8 Article

Cyanines as New Fluorescent Probes for DNA Detection and Two-Photon Excited Bioimaging

Journal

ORGANIC LETTERS
Volume 12, Issue 10, Pages 2194-2197

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ol100474b

Keywords

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Funding

  1. Hong Kong Research Grant Council
  2. GRF
  3. HKBU [202408]
  4. Hong Kong Baptist University
  5. Faculty Research Grant
  6. Research Grants Council of Hong Kong
  7. Consulate General of France in Hong Kong [F-HK04/07T]
  8. [FRG/08-09/11-16]

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A series of cyanine fluorophores based on fused aromatics as an electron donor for DNA sensing and two-photon bioimaging were synthesized, among which the carbazole-based biscyanine exhibits high sensitivity and efficiency as a fluorescent light-up probe for dsDNA, which shows selective binding toward the AT-rich regions. The synergetic effect of the bischromophoric skeleton gives a several-fold enhancement in a two-photon absorption cross-section as well as a 25- to 100-fold enhancement in two-photon excited fluorescence upon dsDNA binding.

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