Journal
ORGANIC LETTERS
Volume 12, Issue 7, Pages 1608-1611Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ol100338v
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Funding
- National Science Council
- Academia Sinica
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The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.
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