Journal
ORGANIC LETTERS
Volume 10, Issue 19, Pages 4175-4178Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ol801582w
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Funding
- La Region Haute-Normandie
- QUIDD
- Institut Universitaire de France (IUF)
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The design and synthesis of novel water-soluble far-red emitting phenol-based fluorophores derived from 7-hydroxycoumarin are described. These hemicyanine-coumarin hybrids display promising spectroscopic features such as large apparent Stokes shift (ranging from 60 to 140 nm) and fluorescence emission maxima between 620 and 720 nm in physiological conditions. Their utility was then illustrated by the preparation of an original fluorogenic probe of penicillin G acylase (PGA) whose fluorescence is unveiled through an enzyme-initiated domino reaction.
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