Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 12, Issue 32, Pages 6128-6133Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4ob00382a
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Funding
- National Natural Science Foundation of China [51273107, 51273175]
- Natural Science Foundation of Shandong Province, China [ZR2012EMZ001]
- Shandong University [2011JC006]
- State Key Laboratory for Supermolecular Structure and Materials [SKLSSM201419, 2013CB834704]
- National Basic Research Program of China (973 Project) [2013CB834702]
- University Grants Committee of HKSAR [AoE/P-03/08]
- Hong Kong Research Grants Council [HKBU203011, HKUST2/CRF/10]
- Hong Kong Baptist University [FRG2/11-12/156]
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Cysteine (Cys) plays important roles in many physiological processes of eukaryotic cells and its detection in cells is of fundamental significance. However, glutathione (GSH), homocysteine, N-acetyl-L-cysteine and other thiols greatly hamper the detection of Cys. In particular, GSH strongly interferes with the detection of cellular Cys (30-200 mu M) due to its high intracellular concentration (1-10 mM). In this work, an off on fluorescent probe (HOTA) for the detection of Cys is presented. This probe possesses both excellent sensitivity and satisfactory selectivity for cellular Cys detection: with the addition of 200 mu M Cys, the fluorescence intensity of the probe (10 mu M) enhanced 117-fold and the detection limit was calculated to be 13.47 mu M, which is lower than the cellular Cys concentration; the probe also selectively detected 30-200 mu M cysteine over 1-10 mM glutathione. Consequently, cell imaging experiments were performed with probe HOTA. Furthermore, the results of the thiol-blocking and GSH synthesis inhibiting experiments confirmed that the intracellular emission mainly originates from the interaction between Cys and HOTA.
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