4.6 Article

A 2,7-carbazole-based dicationic salt for fluorescence detection of nucleic acids and two-photon fluorescence imaging of RNA in nucleoli and cytoplasm

Journal

ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 9, Issue 10, Pages 3615-3618

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/c1ob05123g

Keywords

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Funding

  1. National Science Foundation of China [50673053, 50173015, 30771091, 50990061, 50721002]
  2. NSFC/RGC [50218001]
  3. Open Point of State Key Laboratory of Supramolecular Structure and Materials
  4. Innovation Fund for the Post-Doctoral Program of Shandong Province [201003076]
  5. University Grants Committee of HKSAR, China [AoE/P-03/08]
  6. Hong Kong Baptist University [FRG/08-09/I-20]

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A new carbazole-derived dicationic compound, namely 2,7-bis(1-hydroxyethyl-4-vinylpyridinium iodine)-N-ethyl-carbazole (2,7-9E-BHVC), with a large two-photon action absorption cross section in nucleic acids has been obtained. Moreover, it possesses the potential of imaging RNA in nucleoli and cytoplasm in two-photon fluorescence microscopy and exhibits good counterstain compatibility with the commercial fluorescent nucleic dye DAPI.

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