Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 8, Issue 5, Pages 1194-1201Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b924072a
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Funding
- Academy of Sciences of the Czech Republic [Z4 055 0506]
- Ministry of Education [LC512]
- Czech Science Foundation [203/09/0317]
- Gilead Sciences, Inc. (Foster City, CA, U. S. A.)
- Finnish Ministry of Education
- Finnish Cultural Foundation
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Aqueous Sonogashira cross-coupling reactions of 5-iodopyrimidine or 7-iodo-7-deazaadenine nucleosides with bile acid-derived terminal acetylenes linked via an ester or amide tether gave the corresponding bile acid-nucleoside conjugates. Analogous reactions of halogenated nucleoside triphosphates gave directly bile acid-modified dNTPs. Enzymatic incorporation of these modified nucleotides to DNA was successfully performed using Phusion polymerase for primer extension. One of the dNTPs (dCTP bearing cholic acid) was also efficient for PCR amplification.
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