Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 8, Issue 20, Pages 4601-4606Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0ob00322k
Keywords
-
Categories
Funding
- NIH [R01GM086225, R03MH83266, R21AI090213]
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [ZIBHG200319] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R21AI090213] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM086225, R01GM075797, R01GM100305] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF MENTAL HEALTH [R03MH083266] Funding Source: NIH RePORTER
Ask authors/readers for more resources
In high throughput screening (HTS) campaigns, the quality and cost of commercial reagents suitable for pilot studies often create obstacles upon scale-up to a full screen. We faced such challenges in our efforts to implement HTS for inhibitors of the phosphopantetheinyl transferase Sfp using an assay that had been validated using commercially available reagents. Here we demonstrate a facile route to the synthetic preparation of reactive tetraethylrhodamine and quencher probes, and their application to economically produce fluorescent and quencher-modified substrates. These probes were prepared on a scale that would allow a full, quantitative HTS of more than 350,000 compounds.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available