Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 8, Issue 8, Pages 1849-1860Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b920048g
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Funding
- Spanish Ministerio de Educacion y Ciencia [CTQ2006-15515-C02-01/BQU, CTQ2007-61180/PPQ]
- Junta de Andalucia
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A versatile synthetic procedure to construct series of high- and low-density homo- and heteroglycoclusters is reported. The binding properties of these synthetic multivalent glycoconjugates to concanavalin A (Con A), a model lectin, have been assessed by using a range of competitive and non-competitive binding assays including enzyme-linked lectin assays (ELLA), isothermal titration microcalorimetry (ITC) and surface plasmon resonance (SPR). In all cases, highly dense glycoclusters showed a substantial amplification of the lectin-binding strength in comparison with low-density counterparts. Interestingly, highly-dense glycoligand presentations, regardless of their homo- or heteroglycoligand pattern, furnished similar Con A binding properties, supporting the existence of a synergic effect (heterocluster effect) due to secondary interactions of non-active structural motifs in the presence of a certain density of active glycoligands.
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