Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 8, Issue 7, Pages 1610-1618Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b920443a
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Funding
- Durham County Council STBE One North East
- Durham University
- Engineering and Physical Sciences Research Council [EP/G05021X/1, EP/C508955/1] Funding Source: researchfish
- EPSRC [EP/G05021X/1] Funding Source: UKRI
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A carbamate linked quenching group coupled with a pro-quinone methide reactive core provides an effective tool for studying enzyme function without problems associated with background fluorescence from unreacted probe. However, the relatively slow fragmentation of the carbamate linkage in such a strategy may cause problems of loss of signal or a decoupling of enzyme activity and labelling.
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