Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 6, Issue 12, Pages 2085-2094Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b803895c
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Funding
- Engineering and Physical Sciences Research Council [EP/G004773/1] Funding Source: researchfish
- EPSRC [EP/G004773/1] Funding Source: UKRI
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Five structurally related europium(III) complexes of heptadentate macrocyclic ligands bearing azaxanthone or azathiaxanthone chromophores have been evaluated comparatively as responsive probes of the intracellular environment. Protein binding (HSA) and oxy-anion binding constants have been measured by titrimetric analysis, examining ratiometric emission changes for three examples, and systems exhibiting selectivity for citrate or hydrogencarbonate identified. The cytotoxicity of each ligand and complex has been assessed and correlated with the observed chemical stability pro. le in competitive aqueous media. The europium complex of L-2 is non-toxic, exhibits a large change in its emission spectral pro. le to variation in HCO3- (or citrate) concentration allowing ratiometric analysis, and localises in cellular mitochondria. Such features augur well for its future application as a responsive probe in microscopy to monitor local changes in pHCO(3).
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