Journal
ORAL ONCOLOGY
Volume 45, Issue 10, Pages 887-890Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.oraloncology.2009.03.008
Keywords
Oral potentially malignant disorders; DNA ploidy; Flow cytometry; Tobacco smoking
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Funding
- MURST
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To date there are still no reliable biomarkers for oral potentially malignant disorders (PMDs) to predict the risk of progression to squamous cell carcinoma (SCC). Within a prospective clinical trial of patients with PMDs, DNA content flow cytometry (DNA FCM) was evaluated for 60 PMDs using fresh samples obtained by a dermatological curette. There were 6/42 PMDs without dysplasia, but with DNA aneuploidy, versus 8/18 with both dysplasia and aneuploidy (p = 0.02). When the tongue and the buccal mucosa, the two most common sites in the present series of cases were compared, dysplastic PMDs were mainly located on the tongue (p = 0.01). Tobacco smokers, who preferentially developed PMDs in the buccal mucosa at a younger age than non-smokers (p = 0.002), had fewer dysplastic PMDs than did nonsmokers (p = 0.01). Dysplasia was significantly linked to DNA aneuploidy (p = 0.03) in smokers. The present data suggest that aneuploidy is an early event in oral carcinogenesis and that the influence of tobacco varies according to subsite and patient age. When DNA FCM of PMD samples are obtained by curette scraping, extensive areas can be covered with a minimally invasive, rapid, inexpensive procedure. Moreover DNA FCM of these samples appears easy amenable to routine analysis. Further research on larger numbers of PMDs should be carried out to determine whether DNA FCM plays a role in the prediction of risk of PMD transformation. (C) 2009 Elsevier Ltd. All rights reserved.
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