Journal
ORAL DISEASES
Volume 16, Issue 1, Pages 34-38Publisher
WILEY
DOI: 10.1111/j.1601-0825.2009.01604.x
Keywords
salivary exosomes; microRNA; biomarkers
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Funding
- NIDCR, NIH
- NATIONAL INSTITUTE OF DENTAL & CRANIOFACIAL RESEARCH [ZIADE000733, ZIADE000734, ZIADE000704] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [ZIADK043400] Funding Source: NIH RePORTER
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Objective: The aim of this study was to examine the presence of microRNAs (miRNAs) within exosomes isolated from human saliva and to optimize and test methods for successful downstream applications. Design: Exosomes isolated from fresh and frozen glandular and whole human saliva were used as a source of miRNAs. The presence of miRNAs was validated with TaqMan quantitative PCR and miRNA microarrays. Results: We successfully isolated exosomes from human saliva from healthy controls and a patient with Sjogren's syndrome. microRNAs extracted from the exosomal fraction were sufficient for quantitative PCR and microarray profiling. Conclusions: The isolation of miRNAs from easily and non-invasively obtained salivary exosomes with subsequent characterization of the miRNA expression patterns is promising for the development of future biomarkers of the diagnosis and prognosis of various salivary gland pathologies.
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