Journal
OPTICS LETTERS
Volume 39, Issue 2, Pages 303-306Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.39.000303
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Raman spectroscopy is an established technique for molecularly specific characterization of tissues. However, even with near-infrared (NIR) excitation, some tissues possess background autofluorescence, which can overwhelm Raman scattering. Here, we report collection of spectra from tissues with strong autofluorescence using a 1064 nm system with a high-throughput dispersive spectrometer and deep-cooled InGaAs array. Spectra collected at 1064 nm were compared with those collected at 785 nm in specimens from human breast, liver, and kidney. The results demonstrate superior performance at 1064 nm in the liver and kidney, where NIR autofluorescence is intense. The results indicate the feasibility of new biomedical applications for Raman spectroscopy at 1064 nm in tissues with strong autofluorescence. (C) 2014 Optical Society of America
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